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Amplicon technology: a new way to detect tobacco whitefly resistance

Tobacco whitefly (bemisia tabaci) is a harmful pest distributed globally and can have a serious impact on vegetable production. Its resistance to crop protection is one of the difficulties in practice. Therefore, the detection of the resistance gene mutations can provide an important reference for pest management. However, the individual Bemisia tabaci is small, with a body length of less than 1mm. The traditional single-head sequencing operation is difficult, and often a small amount of gDNA is obtained, but it consumes a lot of time and resources, and new detection methods are urgently needed in scientific research.

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© Tomasz Klejdysz | Dreamstime.com 

The Vegetable Pest Research Laboratory has established a method to detect gene mutation frequencies in micro-insects using amplicon technology and detected the frequency of two pyrethroid resistance-related point mutations of sodium ion channel genes in the Bemisia tabaci population. The method is efficient and reliable and solves the problem of detecting gene mutation frequency of micro-insects.

Amplicon sequencing was originally used to detect the community composition of soil, plant, or animal gut microbes, which can be used to analyze the interaction between microbes and animals and plants. The amplicon sequencing method is based on the Next-generation Sequencing technology, which has high sequencing efficiency and can perform centralized detection of a large number of samples.

The team established an efficient approach for detecting the frequency of mutation by amplicon sequencing. The frequencies of L925I and T929V in VGSC associated with pyrethroid resistance were detected in this study, which could provide foundational data for resistance management of B. tabaci.

This research provides an efficient and reliable method for detecting the frequency of gene mutations in micro-insects and is helpful to the development of pest control in the field. The research was published in the entomology professional journal Pest Management Science (impact factor 3.75), Q1 of the Chinese Academy of Sciences. The first author of the thesis is Wei Yiyun, a postdoctoral researcher at the Lab. Associate researcher Wang Ran, Dr. Qu Cheng, and Dr. Guan Fang from Nanjing Agricultural University participated in part of the work. Researcher Luo Chen is the corresponding author of the paper.

 

Wei, Y., Guan, F., Wang, R., Qu, C. and Luo, C. (2021), Amplicon sequencing detects mutations associated with pyrethroid resistance in Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Manag

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Israel announces creation of global seed company

Two Israeli seed producers, Nirit Seeds and TomaTech, have joined forces to create Israel’s largest vegetable seed company. This synergy will enable unique seed breeding research and development that will accelerate the company’s growth in the international seed markets.

Upon completion of the merger, the company will have more than 80 employees worldwide, including molecular biologists, breeders, agronomists, and others.
The company will improve its breeding activities in Israel, Spain, Mexico and Italy, as well as significantly expand its representation in the Netherlands and North America, with a particular focus on growing vegetables in high-tech greenhouses.

Nirit Seeds is one of the most successful internationally traded tomato and pepper seed producers, investing in the development of revolutionary genetic technologies. TomaTech is one of the leading tomato seed companies in Israel, developing premium varieties, including those resistant to the dangerous Tomato Brown Ruffle Virus (ToBRFV).

The production of crop seeds is one of the most advanced and high-tech sectors in Israel. According to the Seed Department of the Federation of Israel Chambers of Commerce, seed exports currently generate $200 million in annual revenue. There are about 25 large and medium-sized companies in the local industry.

For reference. According to market research, the global vegetable seed industry is valued at $8 billion a year and is growing at a CAGR of 8%.

Prepared according to https://www.freshplaza.com

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Karachay-Cherkessia became one of the leaders in the production of greenhouse vegetables at the end of 2021

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Greenhouse vegetable production in 2021 reached a record 1.4 million tonnes. According to the Ministry of Agriculture of the Russian Federation, the leaders among the regions in this segment are the Karachay-Cherkess Republic, Lipetsk, Moscow, Kaluga, Volgograd, Novosibirsk, Saratov, Chelyabinsk regions, Krasnodar and Stavropol Territories, the Republics of Bashkortostan and Tatarstan. These regions account for more than 60% of the total production in the country. The intensive development of greenhouse projects in our country helps to provide Russians with fresh vegetables all year round. Last year, the harvest in winter greenhouses updated the 2020 record – more than 1.4 million tons of products were received. Including the production of cucumbers amounted to at least 830 thousand tons, and tomatoes – 590 thousand tons. It is expected that by 2025 the volume of vegetable production in year-round greenhouses will be at least 1.6 million tons of vegetables.

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December: Special Year Overview

In 2022, it will be 22 year since the new millennium started. Can’t believe it? Neither can we, but it is true. Before we go there, it is time to reflect on what has happened this year. Over the next couple of weeks, we will look back at 2021 and we will highlight the most important events that impacted the industry.

New greenhouses, events, even a few shows, technical novelties, and all other relevant news will pass by, offering you something other than Covid to talk about during Christmas and New Years’.

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Wish your customers Happy Holidays
This special also offers an opportunity to put your company in the spotlight with a banner in this special box. This banner can be booked until December 24 on our newsletters. For more information, feel free to send an email to: info@hortidaily.com 

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