Connect with us


Model predicts gene responses to cold across diverse plant species

When Xiaoxi Meng and Zhikai Liang first proposed the idea a couple of years ago, James Schnable was skeptical. To say the least.

“‘Well, you can try, but I don’t think it’s going to work,’” the associate professor of agronomy and horticulture recalled saying to Meng and Liang, then postdoctoral researchers in Schnable’s lab at the University of Nebraska–Lincoln.

He was wrong and, in hindsight, never happier to be. Yet at the time, Schnable had fair reason to raise an eyebrow. The duo’s idea — that the DNA sequences of cold-sensitive crops that surrender to a hard frost could help predict how wilder, hardier plants tolerate freezing conditions — seemed audacious. To say the least. Still, it was a low-risk, high-reward proposition. Because if Meng and Liang could get it to work, it might just fast-track efforts to make cold-sensitive crops a little or even a lot more like their cold-resistant counterparts.

Some of the world’s most important crops were domesticated in tropical regions — corn in southern Mexico, sorghum in eastern Africa — that put no selective pressure on them to evolve defenses against cold or freezing. When those crops are grown in harsher climates, their sensitivity to cold limits how early they can be planted and how late they can be harvested. Shorter growing seasons equal less time for photosynthesis, resulting in smaller yields and less food for a global population expected to approach 10 billion people by 2050.

Cold climates
Plant species that already grow in colder climates, meanwhile, evolved tricks to endure the cold. They can reconfigure their cellular membranes to maintain liquidity at lower temperatures, preventing the membranes from freezing and fracturing. They can add dashes of sugars to the liquids in and around those membranes, lowering their freezing point in much the same way that salt does a sidewalk’s. They can even produce proteins that smother minuscule ice crystals before those crystals grow into cell-busting masses.

All of those defenses originate at the genetic level, though not just in the sequences of DNA itself. When plants begin to freeze, they can respond by essentially turning certain genes off or on — preventing or allowing their genetic instruction manuals to be transcribed and carried out. Knowing which genes cold-tolerant plants turn off and on in the face of freezing temperatures, then, can help researchers grasp the very foundations of their fortifications and, ultimately, engineer similar defenses into cold-sensitive crops.

But Schnable also knew, as Meng and Liang did, that even an identical gene often responds differently to cold across plant species, even closely related ones. Which means, frustratingly, that understanding how a gene responds to cold in one species tends to tell plant scientists almost nothing conclusive about the gene’s behavior in another. That unpredictability, in turn, has hindered efforts to learn the rules dictating what will deactivate or activate genes.

“We’re still really, really bad at understanding why genes turn off and on,” Schnable said.

Corn plants
Lacking a rulebook, the researchers turned to machine learning, a form of artificial intelligence that can essentially write its own. They specifically developed a supervised classification model — the sort that can, when presented with enough labeled images of, say, cats and not-cats, eventually learn to distinguish the former from the latter. The team initially presented its own model with an enormous pile of sequenced genes from corn, along with the average activity levels of those genes when the plant was subjected to freezing temperatures. The model was also fed “every feature we could think of” for each corn gene, Schnable said, including its length, its stability and any differences between it and other versions of it found in other corn plants.

Later, the researchers tested their model by concealing from it just one piece of information in a subset of those genes: whether they responded to the onset of freezing temperatures, or whether they didn’t. By analyzing the features of genes it had been told were either responsive or non-responsive, the model discerned which combinations of those features were relevant to each — and then successfully slotted the majority of the remaining, mystery-box genes into their correct categories.

It was a promising start, no doubt. But the real test remained: Could the model take the training it had received in one species and apply it to another?

The answer was a definitive yes. After being trained with DNA data from just one of six species — corn, sorghum, pearl millet, proso millet, foxtail millet or switchgrass — the model was generally able to predict which genes in any of the other five would respond to freezing. To Schnable’s surprise, the model held up even when it was trained on a cold-sensitive species — corn, sorghum, pearl or proso millet — but tasked with predicting gene responses in the cold-tolerant foxtail millet or switchgrass.

“The models we trained worked almost as well across species as if you actually had data in one species and used the internal data to make the predictions in that same species,” he said, a hint of wonder lingering in his voice months later. “I really would not have predicted that.”

“The idea that we can just feed all of this information into a computer, and it can figure out at least some rules to make predictions that work, is still kind of amazing to me.”

Those predictions could prove especially useful when considering the alternative. For roughly a decade, plant biologists have actually been able to measure the number of RNA molecules — the ones responsible for transcribing and transporting DNA instructions — produced by every gene in a living plant. But comparing how that gene expression responds to cold in living specimens, and across multiple species, is a painstaking undertaking, Schnable said. That’s particularly true with wild plants, whose seeds can be difficult to even acquire. Those seeds may not germinate when expected, if at all, and can take years to grow. Even if they do, every resulting plant has to be cultivated in an identical, controlled environment and studied at the same developmental stage.

More species
All of that poses a massive challenge to growing enough wild specimens, from enough wild species, to replicate and statistically evaluate their genes’ responses to cold.

“If we really want to get at what genes are important — that actually play a role in how the plant adapts to cold — we need to be looking at more than two species,” Schnable said. “We want to look at a group of species that are tolerant of cold and a group that are sensitive, and look at the patterns: “This same gene always responds in one and always doesn’t respond in the other.”

“That starts to become a really big and expensive experiment. It’d be really nice if we could just make predictions from the DNA sequences of those species instead of, say, taking 20 species and trying to get all of them at the same stage, put them all through the exact same stress treatments, and measure the amount of RNA produced for each gene in each species.”

Fortunately for the model, researchers have already sequenced the genomes of more than 300 plant species. An ongoing international effort could push that number as high as 10,000 over the next few years.

Though the model has already wildly exceeded his modest expectations, Schnable said the next step will nevertheless involve “convincing both ourselves and other people” that it works as well as it has so far. In every test case to date, the researchers have asked the model to tell them what they already knew. The ultimate test, he said, will come when both the humans and the machine are starting from scratch.

“The next big experiment I think we need to do is to make predictions on a species where we don’t have any data at all,” he said. “To convince people that it really works in cases where even we don’t know the answers.”

The team reported its findings in the journal Proceedings of the National Academy of Sciences. Meng, Liang and Schnable authored the study with Nebraska’s Rebecca Roston, Yang Zhang, Samira Mahboub and undergraduate student Daniel Ngu, along with Xiuru Dai, a visiting scholar from Shandong Agricultural University.

For more information:
University of Nebraska Lincoln

Continue Reading


Israel announces creation of global seed company

Two Israeli seed producers, Nirit Seeds and TomaTech, have joined forces to create Israel’s largest vegetable seed company. This synergy will enable unique seed breeding research and development that will accelerate the company’s growth in the international seed markets.

Upon completion of the merger, the company will have more than 80 employees worldwide, including molecular biologists, breeders, agronomists, and others.
The company will improve its breeding activities in Israel, Spain, Mexico and Italy, as well as significantly expand its representation in the Netherlands and North America, with a particular focus on growing vegetables in high-tech greenhouses.

Nirit Seeds is one of the most successful internationally traded tomato and pepper seed producers, investing in the development of revolutionary genetic technologies. TomaTech is one of the leading tomato seed companies in Israel, developing premium varieties, including those resistant to the dangerous Tomato Brown Ruffle Virus (ToBRFV).

The production of crop seeds is one of the most advanced and high-tech sectors in Israel. According to the Seed Department of the Federation of Israel Chambers of Commerce, seed exports currently generate $200 million in annual revenue. There are about 25 large and medium-sized companies in the local industry.

For reference. According to market research, the global vegetable seed industry is valued at $8 billion a year and is growing at a CAGR of 8%.

Prepared according to

Continue Reading


Karachay-Cherkessia became one of the leaders in the production of greenhouse vegetables at the end of 2021


Greenhouse vegetable production in 2021 reached a record 1.4 million tonnes. According to the Ministry of Agriculture of the Russian Federation, the leaders among the regions in this segment are the Karachay-Cherkess Republic, Lipetsk, Moscow, Kaluga, Volgograd, Novosibirsk, Saratov, Chelyabinsk regions, Krasnodar and Stavropol Territories, the Republics of Bashkortostan and Tatarstan. These regions account for more than 60% of the total production in the country. The intensive development of greenhouse projects in our country helps to provide Russians with fresh vegetables all year round. Last year, the harvest in winter greenhouses updated the 2020 record – more than 1.4 million tons of products were received. Including the production of cucumbers amounted to at least 830 thousand tons, and tomatoes – 590 thousand tons. It is expected that by 2025 the volume of vegetable production in year-round greenhouses will be at least 1.6 million tons of vegetables.


Continue Reading


December: Special Year Overview

In 2022, it will be 22 year since the new millennium started. Can’t believe it? Neither can we, but it is true. Before we go there, it is time to reflect on what has happened this year. Over the next couple of weeks, we will look back at 2021 and we will highlight the most important events that impacted the industry.

New greenhouses, events, even a few shows, technical novelties, and all other relevant news will pass by, offering you something other than Covid to talk about during Christmas and New Years’.


Wish your customers Happy Holidays
This special also offers an opportunity to put your company in the spotlight with a banner in this special box. This banner can be booked until December 24 on our newsletters. For more information, feel free to send an email to: 

Continue Reading