The cultivation of tomatoes holds significant agricultural importance worldwide, facing numerous challenges posed by various biotic and abiotic factors throughout the production process (Panno et al., 2021). Among these factors, viruses, fungi, bacteria, and insects are commonly encountered in greenhouse-grown vegetables.
Employing sensitive, rapid, and cost-effective detection methods is imperative for mitigating such losses in agricultural production. Taqman® probe-based real-time multiplex PCR method has been developed, and this is capable of concurrently detecting three major plant viruses: Tomato brown rugose fruit virus (ToBRFV), tomato spotted wilt orthotospovirus (TSWV), and pepino mosaic virus (PepMV), all of which are prominent viral pathogens affecting tomato production. The multiplex RT-qPCR experiment yielded successful results, with Ct values of 29.34, 25.93, and 27.47 for ToBRFV (at 10−6 dilution), TSWV (at 10−2 dilution), and PepMV (at 10−3 dilution), respectively. Developed primers facilitate the early detection of destructive pathogens ToBRFV, TSWV, and PepMV using both RT-PCR and RT-qPCR, offering a user-friendly and cost-effective approach.
Reference: Cayak, H. N., & Fidan, H. (2024). Multiplex PCR methods for simultaneous detection of tomato brown rugose fruit virus, tomato spotted wilt virus and pepino mosaic virus. Journal of Phytopathology, 172, e13327. https://doi.org/10.1111/jph.13327
